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Protocols

These Center for Biological Imaging protocols and training PDFs are for general reference ONLY!  Please contact a CBI staff member with any questions about your secific experiment and they can provide a protocol and training specific to your project.

protocols:

Cells Immunofluorescence protocol

Immunofluorescence staining protocol for Cells

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Tissue Immunofluorescence protocol

Immunofluorescence staining protocol for Tissue

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Tissue Standard TEM Protocol

Standard EPON embedded TEM protocol for a vriety of tissues.

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Cells Standard TEM Protocol

Standard EPON embedded TEM protocol for Monolayer cells.

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Standard Tissue/Cells SEM protocol

Standard SEM protocol for both tissue and cells.

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LR White TEM protocol

LR White TEM embedding process for gold labeling.

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Immunoelectron TEM protocol

Cryo-sectioning and Immunoelecrton preparation for both tissue and cells.

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General Clearing Protocols

A collection of Clearing protocols we use for deep tissue imaging

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Cytospin Protocol

Protocol for spinning cells onto a slide

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Saponin Protocol

Saponin Immuno Protocol

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Perfusion of Tissue Fixation Protocol

Protocol for tissue perfusiion fixation

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Tissue Fixation and Freezing for IF

Protocol for IF Tissue fixation and freezing

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rECIPES:

PFA Stock Recipe (8%)

Recipe for mixing up PFA fixitive stock solution

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Gelvatol Recipe

Recipe for mixing up your own Gelvitol mounting media

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Hoechst Recipe (DAPI)

Recipe for mixing up Hoechst Nuclear Stain (DAPI)

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equipment tRAINING:

Cryostat Usage Policy

Public access Cryostats require training and a list of materials you will need to bring.

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Nikon NI-E Training

PPT for training on the Nikon NI-E.

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Provis Brightfield Training

PDF of the Provis Brightbield training

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Provis Fluorescent Training

PDF for Provis fluorescent training

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Olympus Fluoview-1000 Training

PDF for the Olympus confocal platform

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